Axoneme-specific β-tubulin specialization

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Axoneme-specific β-tubulin specialization a conserved C-terminal motif specifies the central pair

Axonemes are ancient organelles that mediate motility of cilia and flagella in animals, plants, and protists. The long evolutionary conservation of axoneme architecture, a cylinder of nine doublet microtubules surrounding a central pair of singlet microtubules, suggests all motile axonemes may share common assembly mechanisms. Consistent with this, alpha- and beta-tubulins utilized in motile ax...

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Conserved axoneme symmetry altered by a component β-tubulin

Ninefold microtubule symmetry of the eukaryotic basal body and motile axoneme has been long established [1–3]. In Drosophila, these organelles contain distinct but similar β-tubulin isoforms [4–10]: basal bodies contain only β1-tubulin, and only β2-tubulin is used for assembly of sperm axonemes. A single α-tubulin functions throughout spermatogenesis [11,12]. Thus, differences in organelle asse...

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Axoneme β-Tubulin Sequence Determines Attachment of Outer Dynein Arms

Axonemes of motile eukaryotic cilia and flagella have a conserved structure of nine doublet microtubules surrounding a central pair of microtubules. Outer and inner dynein arms on the doublets mediate axoneme motility [1]. Outer dynein arms (ODAs) attach to the doublets at specific interfaces [2-5]. However, the molecular contacts of ODA-associated proteins with tubulins of the doublet microtub...

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A Direct Interaction between Leucine-rich Repeat Kinase 2 and Specific β-Tubulin Isoforms Regulates Tubulin Acetylation*

Mutations in LRRK2, encoding the multifunctional protein leucine-rich repeat kinase 2 (LRRK2), are a common cause of Parkinson disease. LRRK2 has been suggested to influence the cytoskeleton as LRRK2 mutants reduce neurite outgrowth and cause an accumulation of hyperphosphorylated Tau. This might cause alterations in the dynamic instability of microtubules suggested to contribute to the pathoge...

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Conserved and specific functions of axoneme components in trypanosome motility.

The Trypanosoma brucei flagellum is unusual as it is attached along the cell body and contains, in addition to an apparently conventional axoneme, a structure called the paraflagellar rod, which is essential for cell motility. Here, we investigated flagellum behaviour in normal and mutant trypanosome cell lines where expression of genes encoding various axoneme proteins (PF16, PF20, DNAI1, LC2)...

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ژورنال

عنوان ژورنال: Current Biology

سال: 2001

ISSN: 0960-9822

DOI: 10.1016/s0960-9822(01)00150-6